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BioMed Research International
Volume 2013, Article ID 340626, 8 pages
Research Article

Protective Effect of Fermented Soybean Dried Extracts against TPA-Induced Oxidative Stress in Hairless Mice Skin

1Department of Pharmaceutical Science, Health Sciences Center, Londrina State University (UEL), Avenue Robert Koch 60, 86038350 Londrina, PR, Brazil
2Department of Pharmaceutical Science, Faculty of Pharmaceutical Sciences of Ribeirao Preto (USP), Avenue Do Café s/n, 14040903 Ribeirao Preto, SP, Brazil
3Department of Pathology, Biological Sciences Center, Londrina State University (UEL), Rodovia Celso Garcia Cid KM380 PR445, Campus Universitário, Caixa Postal 6001, 86051970 Londrina, PR, Brazil

Received 18 April 2013; Revised 3 July 2013; Accepted 17 July 2013

Academic Editor: Ajit S. Narang

Copyright © 2013 Sandra R. Georgetti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study evaluated the chemical properties (polyphenol and genistein contents) of soybean extracts obtained by biotransformation and dried by spray dryer at different conditions and their in vivo ability to inhibit 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced biochemical alterations in the skin of hairless mice. By comparing the obtained data with that of the well-known active soybean extract Isoflavin beta, we evaluated the influence of the fermentation and drying process in the extracts efficacy. The results demonstrated that inlet gas temperature and adjuvant concentration for the extract drying process have significantly affected the total polyphenol contents and, to a minor degree, the genistein contents. However, the effect of topical stimulus with TPA, an oxidative stress inducer, which caused significant depletion of reduced glutathione (GSH) and catalase, with increased levels of H2O2 and lipid peroxidation (MDA) in the skin of hairless mice, was significantly prevented by the soybean extracts treatment. These results indicate that the spray drying processing resulted in a product capable of limiting the oxidative stress with possible therapeutic applicability as an antioxidant in pharmaceutical forms.