Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix

Figure 5

Expression of mesenchymal stem cell-related surface marker proteins in BM-MSCs on culture dishes coated without or with 1% (wt/v) gelatin by passage 5. Cultured BM-MSCs were stained with fluorescence-conjugated primary antibodies against CD90 and CD29 (mesenchymal stem cell-specific markers), CD45 (hematopoietic stem cell-specific marker), and CD31 (endothelial cell-specific marker) and analyzed using flow cytometry. No significant differences in the expression of BM-MSC-specific, hematopoietic stem cell-specific, and endothelial cell-specific surface marker proteins were induced by gelatin. All data shown are means ± S.D. of three independent experiments.