Effects of Flavonoids on Rumen Fermentation Activity, Methane Production, and Microbial Population

Table 1

PCR primer sets used in this study*.

Microorganism	Forward	Reverse	Amplicon size (bp)	Reference
General bacteria	cggcaacgagcgcaaccc	ccattgtagcacgtgtgtagcc	130	[45]
General fungi	gaggaagtaaaagtcgtaacaaggtttc	caaattcacaaagggtaggatgatt	120	[45]
Total protozoa	gctttcgwtggtagtgtatt	cttgccctcyaatcgtwct	223	[46]
Total methanogens	cgwagggaagctgttaagt	taccgtcgtccactcctt	343	[47]
Ruminococcus albus	ccctaaaagcagtcttagttcg	cctccttgcggttagaaca	175	[48]
Ruminococcus flavefaciens	cgaacggagataatttgagtttacttagg	cggtctctgtatgttatgaggtattacc	132	[45]
Fibrobacter succinogenes	gttcggaattactgggcgtaaa	cgcctgcccctgaactatc	121	[45]

∗Primer sequence (5′→3′).