Differential Regulation of Ferritin Subunits and Iron Transport Proteins: An Effect of Targeted Hepatic X-Irradiation

Figure 4

qRT-PCR analysis of total RNA from isolated rat hepatocytes treated with irradiation 8 Gy, alone or in combination with major acute phase cytokines (IL-6, IL-1β and TNF-α). Data are shown as fold changes in mRNA expression of iron storage proteins (FTL and FTH) at various time points relative to untreated controls for each time point. qRT-PCR was normalized by using two housekeeping genes: β-actin and ubiquitin C. Results represent means ± SEM of three experiments; ^∗𝑃<0.05, 𝑛=3.