Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in Sugarcane
Figure 2
Products amplified by LAMP reactions with different concentration ratios between inner and outer primers. (a) Detection of LAMP products by color change. Samples turning yellowish green were considered positive, while the ones remaining orange were considered negative. (b) Agarose gel electrophoresis visualization of the LAMP products. Lane M, marker; lanes 1, 6, and 11: the sterilized ddH2O; lanes 2, 7, and 12: the negative DNA extracted from the internode juice without Lxx; lanes 3, 8, and 13: 100 ng/μL positive plasmid; lanes 4, 9, and 14: 50 ng/μL positive plasmid; lanes 5, 10, and 15: 20 ng/μL positive plasmid; lanes 1–5, the concentration ratio of 4 : 1 between inner and outer primers; lanes 6–10, the concentration ratio of 8 : 1 between inner and outer primers; lanes 11–15, the concentration ratio of 10 : 1 between inner and outer primers.
