Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in Sugarcane
Figure 3
Products amplified by LAMP reactions with different Mg2+ concentrations. (a) Detection of LAMP products by color change. Samples turning yellowish green were considered positive, while the ones remaining orange were considered negative. (b) Agarose gel electrophoresis visualization of the LAMP products. Lane M, marker; lanes 1, 2: the sterilized ddH2O; lanes 3, 4: 20 ng/μL negative DNA extracted from the internode juice without Lxx; lanes 5, 6: 20 ng/μL positive DNA extracted from the Lxx-infected juice; lanes 7, 8: 20 ng/μL positive plasmid.
