Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in Sugarcane
Figure 4
Agarose gel electrophoresis visualization of PCR and LAMP products. PCR(A) and PCR(B), Lane M is 100 bp marker; LAMP(A) and LAMP(B), Lane M is marker. Lanes 1, 10: the sterilized ddH2O; lane 2, the negative DNA extracted from the internode juice without Lxx; lane 11, the negative DNA extracted from the leaf midrib without Lxx; lanes 3–9, the amplification products of 100–10−6 concentration gradient of positive DNA extracted from the Lxx-infected internode juice; lanes 12–18, the amplification products of 100–10−6 concentration gradient of positive DNA extracted from the Lxx-infected leaf midrib.
