Research Article

Development of Loop-Mediated Isothermal Amplification for Detection of Leifsonia xyli subsp. xyli in Sugarcane

Table 1

Sequences of LAMP primers used for detection of Lxx. The “c” indicates the reverse and complementation of the “F1” or “B2” sequence shown in Figure 1.

PrimerSequence (5′-3′)

F3ACATCGGTACGACTGGGT
B3TGGCCGACCAAAAAAGGT
FIP (F1c + F2)GGCGTACTAAGTTCGAGCCGTT-GGTCAGCTCATGGGTGGA
BIP (B1 + B2c)CCTCGCACATGCACGCTGTT-CTCAGCGTCTTGAAGACACA