Regulation of PKC Autophosphorylation by Calponin in Contractile Vascular Smooth Muscle Tissue
Calponin increases autophosphorylation on the PDK and hydrophobic sites but not the turn motif site of recombinant PKC. (a) Mean densitometric results of 6 experiments using a site-specific phosphoantibody to the PDK site (T497) as described in methods. (b) Mean densitometric results of 6 experiments, phosphorylation detected with a site-specific phosphor-antibody to the hydrophobic site (S657). (c) Mean densitometric results of 6 experiments, phosphorylation detected with a site-specific antibody for the turn motif site (T638). No ATP and BSA lanes represent negative controls. Protamine is a positive control. Data are mean ± SE. * and ** versus No ATP.