Regulation of PKC Autophosphorylation by Calponin in Contractile Vascular Smooth Muscle Tissue
Figure 1
Calponin increases autophosphorylation on the PDK and hydrophobic sites but not the turn motif site of recombinant PKC. (a) Mean densitometric results of 6 experiments using a site-specific phosphoantibody to the PDK site (T497) as described in methods. (b) Mean densitometric results of 6 experiments, phosphorylation detected with a site-specific phosphor-antibody to the hydrophobic site (S657). (c) Mean densitometric results of 6 experiments, phosphorylation detected with a site-specific antibody for the turn motif site (T638). No ATP and BSA lanes represent negative controls. Protamine is a positive control. Data are mean ± SE. * and ** versus No ATP.
