Regulation of PKC Autophosphorylation by Calponin in Contractile Vascular Smooth Muscle Tissue

Figure 2

(a) Representative immunoblots of PKC𝛼 and PKC𝜀 hydrophobic site phosphorylation, and PDK phosphorylation, in PE-stimulated ferret aorta samples, by using phosphospecific antibodies. (b)–(d) Quantitation of changes of phosphorylation levels of PKC𝛼, PKC𝜀, and PDK after stimulating with PE (10 μM) in ferret aorta. (b) Time course of phosphorylation at hydrophobic site of PKC𝛼 (S657). (c) Time course of phosphorylation at hydrophobic site of PKC𝜀 (S729). (d) Time course of phosphorylation of PDK (S241). Data are mean ± SE; 𝑛=3--7 animals. *𝑃<0.05 and **𝑃<0.01 compared with control (0 minutes).

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