Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells

Figure 1

Murine cardiac endothelial cells. Heart tissue sections staining demonstrated (a–d) colocalization of CD31 and Sca-1, (e–h) CD31 and CD34, and (i–k) CD31 and vWF at endothelial lining layer. (l) Representative photograph of a heart section stained with secondary antibodies only (anti rat Alexa 488 and anti rabbit Alexa 594) and DAPI shows acceptable green and red background fluorescence. (m, n) eNOS and Caveolin-1 highlighted the endothelial layer. (o) alpha-SMA exclusively stained the smooth muscle cells of vessels without overlapping with CD31 which stained endothelial cell layer. (p) Staining of NG2 and Sca-1 showed Sca-1 staining in endothelium of coronary arteries and capillaries whereas NG2 is exclusively expressed by vascular smooth muscle cells. Photographs were taken with Zeiss Axiovert 200 using Axiovision v4.6.3 software. Scale bar = 100 μm.

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