Research Article
Biosynthesis of Osmoregulated Periplasmic Glucans in Escherichia coli: The Phosphoethanolamine Transferase Is Encoded by opgE
Table 1
Strains, plasmids, and primers.
| |
Strain, phage, plasmid, or primers | Relevant genotype and/or phenotype | Source or reference |
| | Escherichia coli | | | | JM83 | ara (lac-pro) thi rpsL 80Δ(lacZ15) | [1] | | NFB216 | JM83 opgH200::Tn10 pyrC46 | [2] | | NFB732 | JM83 opgB214::Tn10 | [3] | | NFB775 | HfrH thi-1 serB28 opgB214::Tn10 dgk-6 relA1 | This study | | NFB1887 | JM83 opgC1::Tn5 | This study | | NFB2228 | JM83 opgB214::Tn10 opgC::Tn5 | This study | | NFB4548 | JM83 opgB214::Tn10 opgC::Tn5 opgE2::cml | This study | | NFB4576 | JM83 opgE2::cml | This study | | NFB2231 | NFB4548/pNF752 | This study |
| | Phage | | | | NK467 | b221 c1
857Oam29 Pam8O rex::Tn5 (KanR) | N. Kleckner |
| | Plasmids | | | | pUC18Not | AmpR | [4] | | pNFCml | AmpR, CmlR | [5] | | pKD46 | AmpR lambda Red recombinase expression | [6] |
| | Primer | | | | Tn5CEcoRI | GTGAATTCACTCCGTTCTCTTGCTCG | This study | | Tn5DHindIII | GGGAAAGCTTCCGTTCAGGACGCTAC | This study | | foropgE | CCTGAATTCCCGGTGTTGGTTACCGCTTC | This study | | revopgE | GGAAAGCTTATGAATGAGTTCAAGAGGTG | This study | | upopgE | GGATCGACGGGATTAGCAAG | This study | | c1 | TTATACGCAAGGCGACAAGG | [6] | | downopgE | ACGACTGGGCAAGCATCTAC | This study | | c2 | GATCTTCCGTCACAGGTAGG | [6] |
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