Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances
Morphology of rat aortic smooth muscle cells on day 5 after seeding on pristine LDPE (pristine), on a tissue culture polystyrene dish (PS), on LDPE irradiated with Ar+ plasma (plasma), on LDPE irradiated with plasma and grafted with glycine (Gly), on polyethyleneglycol (PEG), on bovine serum albumin (BSA), on colloidal carbon particles (C), or on bovine serum albumin and carbon particles (BSA+C). Cell membrane and cytoplasm stained with Texas Red C2-maleimide (red fluorescence), cell nuclei with Hoechst mumber 33342 (blue to white fluorescence). Olympus IX 51 microscope, DP 70 digital camera, obj. 20x, bar = 200 μm.