Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances
Concentration of focal adhesion, cytoskeletal, and contractile proteins in rat aortic smooth muscle cells in 7-day-old cultures on pristine PE (pristine), on PE activated with plasma (plasma), on PE grafted with glycine (Gly), on polyethylene glycol (PEG), on bovine serum albumin (BSA), on colloidal carbon particles (C), or on BSA with C (BSA+C), and on a tissue culture polystyrene dish (PS dish). Measured by ELISA per mg of protein. Means ± SEM from three to seven experiments, each performed in duplicate or in triplicate. Absorbance values were normalized to the values obtained in cell samples from pristine PE, that is, given as a percentage of the values on pristine PE. ANOVA, statistical significance: *,+ compared to the value on pristine PE (Student-Newman Keuls method and Dunnett’s posttest, resp.).