Adhesion, Growth, and Maturation of Vascular Smooth Muscle Cells on Low-Density Polyethylene Grafted with Bioactive Substances

Table 1

Primary antibodies used for immunofluorescence staining (Immf.) and enzyme-linked immunosorbent assay (ELISA) of markers of adhesion and phenotypic maturation of VSMCs.

Antibody against	Developed in, type	Company, Cat. number	Dilution	Incubation
Chicken talin	Mouse, monoclonal	Sigma^a; T 3287	Immf.: 1 : 200; ELISA: 1 : 500	Immf.: overnight, 4°C; ELISA: 60 min, RT^c
Human vinculin	Mouse,; monoclonal	Sigma^a; V9131	Immf.: 1 : 200; ELISA: 1 : 400	Immf.: overnight, 4°C; ELISA: 60 min, RT^c
Recombinant human paxillin	Rabbit,; polyclonal	Chemicon^b; P1093	Immf.: 1 : 200; ELISA: 1 : 400	Immf.: overnight, 4°C; ELISA: 60 min, RT^c
Chicken α-actinin	Rabbit,; polyclonal	Sigma^a; A2543	Immf.: 1 : 200; ELISA: 1 : 500	Immf.: overnight, 4°C; ELISA: 60 min, RT^c
Synthetic peptide of α-smooth muscle actin	Mouse,; monoclonal	Sigma^a; A2547	Immf.: 1 : 200; ELISA: 1 : 400	Immf.: overnight, 4°C; ELISA: 60 min, RT^c
Synthetic peptide of β-actin	Mouse,; monoclonal	Sigma^a; A 5441	Immf.: 1 : 200; ELISA: 1 : 400	Immf.: overnight, 4°C; ELISA: 60 min, RT^c
Human smooth muscle SM1 and SM2 myosin	Mouse,; monoclonal	Sigma^a; M7786	Immf.: 1 : 200; ELISA: 1 : 500	Immf.: overnight, 4°C; ELISA: 60 min, RT^c

aSigma, St. Louis, MO, USA; Czech Dealer: Sigma-Aldrich S.R.O., Prague, Czech Republic.
^bChemicon International Inc., Temecula, CA, USA; Czech dealer: Scintilla S.R.O., Jihlava, Czech Republic.
^cRoom temperature (RT).