A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
Figure 1
Device design and stability of concentration gradients as a function of time. (a) The lower 3 wells are cell and/or source wells for chemokines, and the upper 3 wells serve as reservoirs to maintain equivalent pressures in the lower wells. Depending on experimental design, the side wells or the central well can serve as source wells; cells in a central well can migrate toward different chemokine stimuli on either side, or different cell populations in the side wells can migrate towards a central chemokine stimulus. (b, c) Concentration gradients are shown schematically after the addition of a low molecular weight fluorescent dye indicator to the source well and source reservoir, at either 2 hours (b) or 72 hours (c). (d) Graphical display of concentration gradients from 0 hour through 72 h after fluorescent dye addition to source well and reservoir.
