Direct Effect of Chenodeoxycholic Acid on Differentiation of Mouse Embryonic Stem Cells Cultured under Feeder-Free Culture Conditions

Figure 3

Characteristic changes in CDCA-treated mES cells. mES cells were cultivated with 50 𝜇M, 100 𝜇M, or 200 𝜇M CDCA for 72 h. Subcultured mES cells were treated with 100 𝜇M CDCA for another 72 h. Lineage-specific markers such as nestin and PAX6, 𝛼-smooth muscle actin (𝛼-SMA), and 𝛼-fetoprotein (𝛼-FP). LIF: leukemia inhibitory factor; DMSO: dimethyl sulfoxide; CDCA: chenodeoxycholic acid.