Research Article
Direct Effect of Chenodeoxycholic Acid on Differentiation of Mouse Embryonic Stem Cells Cultured under Feeder-Free Culture Conditions
Table 1
Primer sequences and cycling conditions used for RT-PCR.
| Gene | Primer sequence | Annealing temperature | Product size |
| Oct4
| Forward 5′-GAAGCCCTCCCTACAGCAGA-3′ | 60°C | 297 bp | Reverse 5′-CAGAGCAGTGACGGGAACAG-3′ | Nanog
| Forward 5′-CCCCACAAGCCTTGGAATTA-3′ | 60°C | 255 bp | Reverse 5′-CTCAAATCCCAGCAACCACA-3′ | Nestin
| Forward 5′-TAGAGGTGCAGCAGCTGCAG-3′ | 60°C | 170 bp | Reverse 5′-AGCGATCTGACTCTGTAGAC-3′ | NCAM
| Forward 5′-AGATGGTCAGTTGCTGCCAA-3′ | 60°C | 187 bp | Reverse 5′-AGAAGACGGTGTGTCTGCTT-3′ | α-SMA
| Forward 5′-ACTGGGACGACATGGAAAAG-3′ | 60°C | 240 bp | Reverse 5′-CATCTCCAGAGTCCAGCACA-3′ | Desmin
| Forward 5′-TGACAACCTGATAGACGACC-3′ | 60°C | 180 bp | Reverse 5′-TTAAGGAACGCGATCTCCTC-3′ | α-FP
| Forward 5′-TGCACGAAAATGAGTTTGGGA-3′ | 60°C | 159 bp | Reverse 5′-TTGCAGCCAACACATCGCTA-3′ | Albumin
| Forward 5′-TGCTGCTGATTTTGTTGAGG-3′ | 60°C | 500 bp | Reverse 5′-GCTCACTCACTGGGGTCTTC-3′ | FXR
| Forward 5′-TTGCGACAAGTGACCTCCAC-3′ | 58°C | 653 bp | Reverse 5′-TGATGGTTGAATGTCCGGAG-3′ | GAPDH
| Forward 5′-GTCATCATACTTGGCAGGTT-3′ | 60°C | 489 bp | Reverse 5′-GTCGTGGAGTCTACTGGTGT-3′ |
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NCAM: neural cell adhesion molecule; α-SMA: alpha-smooth muscle actin; α-FP: alpha-fetoprotein; FXR: farnesoid X receptor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.
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