Figure 2: Effect of edaravone on TBI following CCI. (a) Representative core- (upper) and peri-injury (lower) images of FJB staining at 24 hours following CCI. Edaravone was injected 0, 3, or 6 hours following CCI; vehicle treatment occurred at 0 hours. The inset shows a higher power view of the boxed region in the main image. (b) The TBI area was semiquantified for each experimental group. Edaravone ( ) treatment at 3 and 6 hours led to a significant decrease in the core-injury area compared with the vehicle-treated control ( ). The injury volume was calculated by integration of the TBI areas and expressed as total (c) or core- and peri-injury (d) volumes. Data are expressed as mean ± SEM. Asterisk (*) indicates a significant difference between groups based on Tukey-Kramer tests ( values are described in the text).