Salamander-Derived, Human-Optimized nAG Protein Suppresses Collagen Synthesis and Increases Collagen Degradation in Primary Human Fibroblasts

Table 1

Primers and probes used for quantitative real-time PCR.

Gene	Gene accession number	Primer, probe sequence	Product size
Procollagen I	AC number NM_000089.3	Forward: TGG ATT GAC CCT AAC CAA GGA TGC	145
		Reverse: AGA CGT GTT TCT TGT CCT TGG AGC
		Probe: ACT GGC GAA ACC TGT ATC CGG GCC CAA CCT
Procollagen III	AC number NM_000090.3	Forward: AGT CCT GGT GGT AAA GGC GAA ATG	150
		Reverse: TTT GGC ACC ATT CTT ACC AGG CTC
		Probe: AAT GGT GCT CCT GGA CTG CGA GGT GGT GCA
𝛽-actin	AC number NM_001101.3	Forward: ACCGAGCGCGGCTACAG	60
		Reverse: CTTAATGTCACGCACGATTTCC
		Probe: TTCACCACCACGGCCGAGC