BioMed Research International / 2013 / Article / Tab 2

Research Article

Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology

Table 2

EGFR mutation detection in NSCLC samples.

Training setValidation set
SampleSample typeTumor cellsStandard methodsCastPCRMutationSampleSample typeTumor cellsStandard methodsCastPCRMutation

731FFPE (S)*70%WT2826FFPE (S)80%WT
732FFPE (S)50%WT2832FFPE (S)30%WT
828FFPE (S)70%Del15bp_ex192843Cytology300 cellsp.G719A
913aFFPE (B)**30%p.L858R2858FFPE (S)40%p.L858R
966FFPE (S)70%p.L858R2903FFPE (S)30%WT
1070bFFPE (S)90%WT2953Cytology1000 cellsDel9bp_ex19
1232aFFPE (B)60%Del15bp_ex192958FFPE (S)90%p.L858R-p.T790M
1262FFPE (S)40%Del15bp_ex192965FFPE (S)40%WT
1406Cytology1500 cellsWT2971Cytology5000 cellsWT
1591Cytology>500 cellsp.G719S2988FFPE (S)70%WT
1674FFPE (S)50%Del18bp_ex192992FFPE (S)80%Del9bp_ex19
1677FFPE (S)60%L858R3023FFPE (S)60%WT
2139FFPE (S)80%WT3031FFPE (B)20%WT
2355FFPE (S)90%WT3032FFPE (S)20%p.L861Q
2376FFPE (B)60%Del12bp_ex193053FFPE (B)50%WT
2572Cytology300 cellsDel15bp_ex193060FFPE (S)10%WT
2659Cytology5000 cellsWT3070Cytology150 cellsWT
2665Cytology250 cellsWT3089FFPE (B)80%WT
2693Cytology50%WT3098FFPE (S)70%WT
2722FFPE (S)70%Del15bp_ex193111FFPE (B)20%WT
2733Cytology100 cellsWT3140FFPE (S)20%Del18bp_ex19
2739FFPE (B)40%WT3171FFPE (S)60%p.L858R
2762FFPE (B)30%Del15bp_ex193000aFFPE (B)5%Discordant p.L858R
2767FFPE (B)40%p.L858R2768FFPE (B)50%Discordant p.L858R
2778Cytology500 cellsWT1672Cytology500 cellsDiscordant Del18bp_ex19
2787FFPE (S)80%WT2527FFPE (S)70%Discordant Del24bp_ex19
2789FFPE (S)80%p.L858R3014FFPE (S)80%Discordant ins9bp_ex20
2790Cytology250 cellsWT3045FFPE (S)70%Discordant ins3bp_ex20
2798FFPE (S)90%WT
2824FFPE (S)80%WT

FFPE tissue, surgical specimen; FFPE tissue, small biopsy.

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