Lignocellulosic Fermentation of Wild Grass Employing Recombinant Hydrolytic Enzymes and Fermentative Microbes with Effective Bioethanol Recovery
Figure 5
SSF profile of 1% (w/v) wild grass using mixed enzyme mixed culture in shake flask. (●) cell OD measured at 600 nm, (▲) ethanol concentration (g·L−1), (▼) reducing sugar (g·L−1), and (○) specific activity (U·mg−1) with time (h). The cultivation conditions were 100 mL working volume in 250 mL Erlenmeyer flask, initial pH 5.0, temperature 30°C, and shaking 120 rpm. The mixed hydrolytic enzymes were GH5 cellulase with GH43 hemicellulase, and mixed fermentative microbes were S. cerevisiae with C. shehatae. The dynamic profiles of recombinant cellulase (GH5) and hemicellulase (GH43) (data not shown) were similar.
