BioMed Research International / 2013 / Article / Tab 3

Research Article

Lignocellulosic Fermentation of Wild Grass Employing Recombinant Hydrolytic Enzymes and Fermentative Microbes with Effective Bioethanol Recovery

Table 3

Different SSF combinations of recombinant hydrolytic enzymes and fermentative microbes with steam exploded wild grass.

SSF combinationSubstrate concentration (%, w/v) and mode of SSFReducing sugar* (g·L−1)Ethanol yield (g of ethanol·g of substrate−1)Ethanol titre* (g·L−1)

GH5 + S. cerevisiae 1% shake flask1.26 ± 0.060.0670.67 ± 0.02
GH5 + GH43 + S. cerevisiae + C. shehatae (shake flask)1% shake flask1.60 ± 0.050.1281.28 ± 0.03
GH5 + GH43 + S. cerevisiae + C. shehatae (shake flask)5% shake flask13.0 ± 0.040.22411.20 ± 0.06
GH5 + GH43 + S. cerevisiae + C. shehatae (bioreactor)5% bioreactor30.0 ± 0.040.46123.07 ± 0.08

The values correspond to the maximum reducing sugar and maximum ethanol at a particular time; values are mean ± SE ( ).

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