Effect of Folic Acid and Vitamin on Pemetrexed Antifolate Chemotherapy in Nutrient Lung Cancer Cells
Figure 1
Effects of fetal bovine serum (FBS) on MTA sensitivity in human NSCLC cells. Cell cytotoxicity (MTS) assay was performed to measure the sensitivity of cells to MTA (500 nM) under different levels of FBS in culture medium. (a) A549 (400 cells/well), (b) H460 (103 cells/well), and (c) H1299 cells (103 cells/well) were seeded onto 96-well plate for 24 h with 10%, 2%, or 0% (serum-free) serum for 5 days. Paired samples were prepared at each time point, and the survival was calculated by normalizing to the sample without MTA treatment, represented as 100%. The reduced values of survival (% control) indicated the sensitivity of cells to MTA treatment.
