Effect of Folic Acid and Vitamin on Pemetrexed Antifolate Chemotherapy in Nutrient Lung Cancer Cells
Figure 2
Effects of FA/VB12 supplementation on MTA-treated NSCLC cells with 10% serum. Five sets of cells were analyzed on MTS assay to determine cell growth. (a) A549 (400 cells/well), (b) H460 (103 cells/well), and (c) H1299 cells (103 cells/well) were seeded onto 96-well plate for 24 h on day 1 with 10% serum followed by MTA and FA/VB12 treatment. Cells without MTA treatment served as the control (control). The cells were maintained in 10% serum combined with MTA (500 nM, MTA), MTA with 600 nM FA (MTA + FA), MTA with 600 nM VB12 (MTA + VB12), or MTA with FA and VB12 (MTA + FA + VB12) for 5 days. The cell growth ratio was normalized to day 1 of each set of cells. The value was determined by Student’s t-test.
