Figure 1: Phylogenetic relationship and interaction of potato and Arabidopsis PDX proteins. (a) An unrooted phylogenetic tree comparing Arabidopsis and potato PDX1 proteins. Proteins were aligned through Clustal W in a pairwise order.  (b) Y2H results for screens with AtPDX1.3 (upper half) or AtPDX2 (lower half) as baits, respectively. SDII: selection medium for transformation with bait (pBTM116-D9) and prey (pACT2) plasmids supplemented with leucine and histidine; SDIV: selection medium for interaction studies without leucine and histidine supplements. Photos were taken from single spots.