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BioMed Research International
Volume 2013, Article ID 390482, 8 pages
http://dx.doi.org/10.1155/2013/390482
Research Article

High Expression of H3K27me3 Is an Independent Predictor of Worse Outcome in Patients with Urothelial Carcinoma of Bladder Treated with Radical Cystectomy

1State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China
2Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China
3Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, China

Received 23 May 2013; Accepted 31 July 2013

Academic Editor: Xin-yuan Guan

Copyright © 2013 Jianye Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

It has been suggested that trimethylation of lysine 27 on histone H3 (H3K27me3) is a crucial epigenetic process in tumorigenesis. However, the expression pattern of H3K27me3 and its clinicopathological/prognostic significance in urothelial carcinoma of bladder (UCB) are unclear. In this study, upregulated expression of H3K27me3 protein was observed in the majority of UCBs by Western blotting. High expression of H3K27me3 was examined by IHC in 59/126 (46.8%) of UCB tissues and in 18/72 (25.0%) of normal urothelial bladder epithelial tissues ( ). High expression of H3K27me3 was associated with multifocal tumors and lymph node metastases ( ). Patients with high expression of H3K27me3 had shorter cancer-specific survival (CSS) time than patients with low expression of H3K27me3 ( ). In different subsets of UCB patients, high expression of H3K27me3 was also a prognostic indicator in patients with grade 2 and grade 3, pT1, pT2, pT3, and pN− disease ( ). Importantly, expression of H3K27me3 was an independent predictor for CSS ( ) of UCB patients treated with radical cystectomy (RC). Our data suggests that high expression of H3K27me3 is an independent molecular marker for predicting poor prognosis of UCB patients treated with RC.