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BioMed Research International
Volume 2013, Article ID 391549, 6 pages
Research Article

Evaluation of hsp65 Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country

1Post Graduation Program in Biosciences Applied to Pharmacy, Department of Clinical Analyses and Biomedicine, State University of Maringa, 87020-900 Maringá, PR, Brazil
2School of Pharmaceutical Sciences, Department of Biological Sciences, Paulista State University, Araraquara, 14800-901 São Paulo, SP, Brazil
3Department of Clinical Analyses and Biomedicine, State University of Maringa, 87020-900 Maringá, PR, Brazil
4Center of Medical and Pharmaceutical Sciences, State University of Western of Parana, 85819-110 Cascavel, PR, Brazil
5School of Pharmaceutical Science, University of São Paulo, 05508-900 São Paulo, SP, Brazil

Received 22 May 2013; Accepted 8 September 2013

Academic Editor: Andrea Savarino

Copyright © 2013 Sara Macente et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Current study evaluated the hsp65 Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65 Nested PCR-PRA) to detect and identify Mycobacterium tuberculosis complex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB). hsp65 Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. The hsp65 Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively. hsp65 Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country.