Promotion of Hepatic Differentiation of Bone Marrow Mesenchymal Stem Cells on Decellularized Cell-Deposited Extracellular Matrix
Figure 1
The protocol of preparing decellularized ECM. Conventional TCPS flasks were pretreated with gelatin, glutaraldehyde, and ethanolamine. L-ascorbic acid phosphate was supplemented to increase ECM production by BM-MSCs. ECM was decellularized by treating with Triton X-100 and NH4OH.
