Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream

Figure 2

Chromatograms acquired at 280 nm  by HPLC-DAD-UV. (a) Chromatogram of the crude extract without fractionation process. (b) Chromatogram of the residue of the crude extract that remains in the extraction cell after the fractionation process (E_Cell). (c) Chromatogram of the fraction obtained under optimal conditions (E₁₄₀).

(a)

(b)

(c)