Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream

Table 1

SFE operational conditions tested.

Assay	𝑇 (°C)	Time (min)	CO2 flow (g min^−1)	Pressure (bar)	Modifier	Modifier (%)	Modifier flow (mL min^−1)	Fractions obtained
					None	None	None	A100, A120, A140, A160, A200, A250, A300
1	40	30	—*	100, 120, 140, 160, 200, 250, 300	Ethanol	—*	0.1	B100, B120, B140, B160, B200, B250, B300
						—*	0.2	C100, C120, C140, C160, C200, C250, C300
2	40	30	3	100, 120, 140, 160, 200, 250, 300	Ethanol	3; 6	0.1; 0.2	D100, D120, D140, D160, D200, D250, D300; E100, E120, E140, E160, E200, E250, E300
3	40	30	3	140	Ethanol; Methanol	0, 0.5, 1, 1.5, 2, 2.5, 3	0.0, 0.02, 0.03, 0.05, 0.06, 0.08, 0.1	F0, F0.5, F1, F1.5, F2, F2.5, F3; G0, G0.5, G1, G1.5, G2, G2.5, G3

∗In pressure mode, neither CO₂ flow nor modifier percentage were controlled.