Research Article
Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream
Table 2
Recovery yield, radical scavenging activity (DPPH), and colouration of each fraction (Fr.) obtained by SPE with different % of methanol.
| Sample | % of methanol | Recovery yield (% w/w)a | DPPH (EC50)b | Colour |
| Fr. 1 | 0 | 10.4 | 0.44 | Wheat | Fr. 2 | 10 | 18.1 | 0.30 | Burnt orange | Fr. 3 | 20 | 16.2 | 0.27 | Brown | Fr. 4 | 30 | 19.4 | 0.20 | Maroon | Fr. 5 | 40 | 18.3 | 0.26 | Dark brown | Fr. 6 | 50 | 8.19 | 0.23 | Dark brown | Fr. 7 | 60 | 6.71 | 0.89 | Ochre | Fr. 8 | 70 | 1.59 | 7.02 | Colourless | Fr. 9 | 80 | 0.324 | 6.64 | Colourless | Fr. 10 | 90 | 0.615 | 8.25 | Colourless | Fr. 11 | 100 | 0.194 | 14.3 | Colourless | Crude extract | — | — | 0.32 | Dark brown | BHA | — | — | 0.24 | — | BHT | — | — | 2.67 | — |
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aValues expressed as % of dry crude extract.
bValues expressed as g L−1 of extract (fraction).
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