Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream

Table 4

(a) SFE yield determined gravimetrically (w/w) and colour of fractions A, B, and C. (b) SFE yield determined gravimetrically (w/w) and colour of fractions D and E. (c) SFE yield determined gravimetrically (w/w) and colour of fractions F and G.

(a)  

𝑃 (bar)	A		B		C
	Yield (%)	Colour	Yield (%)	Colour	Yield (%)	Colour
100	0.02	Colourless	0.24	Wheat	0.02	Colourless
120	0.02	Colourless	7.71	Dark brown	23.04	Dark brown
140	0.02	Colourless	4.27	Burnt orange	6.39	Brown
160	0.00	Colourless	2.77	Burnt orange	0.06	Wheat
200	0.02	Colourless	12.7	Maroon	0.01	Colourless
250	0.02	Colourless	8.09	Dark brown	0.07	Ochre
300	0.02	Colourless	3.29	Maroon	0.03	Colourless
Total	0.12%		39.1%		29.6%

(b)  

𝑃 (bar)	D		E
	Yield (%)	Colour	Yield (%)	Colour
100	0.89	Brown	3.27	Burnt orange
120	0.73	Brown	19.83	Dark brown
140	0.76	Brown orange	5.67	Burnt orange
160	1.42	Burnt orange	3.65	Burnt orange
200	1.13	Burnt orange	3.84	Burnt orange
250	0.79	Burnt orange	2.56	Burnt orange
300	0.98	Burnt orange	2.64	Burnt orange
Total	6.7%		41.5%

(c)  

% Modifier	F		G
	Yield (%)	Colour	Yield (%)	Colour
0	0.04	Orange	0.13	Wheat
0.5	0.07	Coral	0.08	Wheat
1.0	0.33	Wheat	0.31	Wheat
1.5	0.58	Wheat	0.28	Wheat
2	0.44	Wheat	0.58	Wheat
2.5	0.61	Wheat	0.41	Wheat
3	1.08	Wheat	0.59	Wheat
Total	3.15%		2.38%