Figure 2: Gel-filtration analysis of curcumin-treated PMPMEase. PMPMEase was preincubated with 10 μM curcumin followed by gel-filtration chromatography to separate free curcumin from the enzyme. Aliquots of the collected fractions were assayed for residual PMPMEase activity (a). Michaelis-Menten kinetics (b) and double reciprocal analyses of the inhibition of PMPMEase by curcumin (c). Curcumin treatment (closed circles ●) was compared to untreated control (open circles ○).