Characterization of the Bacterial Community Associated with Larvae and Adults of Anoplophora chinensis Collected in Italy by Culture and Culture-Independent Methods

Table 2

Bacterial taxa identified in larvae and adults of Anoplophora chinensis by culture and culture-independent methods.

Phylum	Class	Family	Genus^a	Larval gut			Adult gut			Testicles and eggs^d
				Detection strategy			Detection strategy			Detection strategy
				Isolation; (al)^c	Library; (al)^c	DGGE^b (li, sa)^c	Isolation; (ac, al)^c	Library; (al)^c	DGGE^b (ac, al)^c	Isolation; (ac, al)^c	DGGE^b (ac, al)^c
Proteobacteria	Alfaproteobacteria	Caulobacteraceae	Brevundimonas	1			1 ac		ac
		Sphingomonadaceae	Sphingomonas						ac
			Novosphingobium	1
		Phyllobacteriaceae	Unclassified	1
		Methylobacteriaceae	Methylobacterium			li			ac
	Betaproteobacteria	Comamonadaceae	Comamonas							1T ac
		Neisseriaceae	Neisseriae		2
		Oxalobacteraceae	Massilia			li			ac
			Ralstonia			li			ac
	Gammaproteobacteria	Enterobacteriaceae	Enterobacter		4		3 ac, 6 al*	80	ac, al	3T ac, 3E ac	E ac, T ac, al
			Klebsiella				8 ac, 2 al		ac, al	1T ac, 4T al	E ac, T ac, al
			Raoultella		1	sa	5 ac, 1 al	58	ac, al	3T al	E ac, T ac, al
			Gibbsiella	7	58	li, sa
			Rahnella			li
			Erwinia				2 ac	1
			Buttiauxella								T ac
			Unclassified	3	16	li, sa	2 ac	2	ac	3T ac	T ac
		Xanthomonadaceae	Stenotrophomonas					1		1T al, 1E ac
			Dyella	1
		Sinobacteraceae	Nevskia						ac
		Pseudomonadaceae	Pseudomonas		1	sa		1		1E ac
		Moraxellaceae	Acinetobacter		2		1 ac	3
Actinobacteria	Actinobacteria	Microbacteriaceae	Microbacterium	1	3	li, sa	2 ac	1	ac
			Curtobacterium							1T ac
		Dermabacteraceae	Brachybacterium					1
		Brevibacteriaceae	Brevibacterium				1 ac, 1 al	1
		Propionibacteriaceae	Propionibacterium		1
		Micrococcaceae	Rothia		1
			Kocuria		1
		Tsukamurellaceae	Tsukamurella				2 ac			1T ac
Firmicutes	Bacilli	Bacillaceae	Bacillus	3	1			2
		Paenibacillaceae	Paenibacillus				1 ac
		Enterococcaceae	Enterococcus			li				1T al
		Streptococcaceae	Streptococcus	2
		Staphylococcaceae	Staphylococcus	1						1E ac
		Planococcaceae	Lysinibacillus							1E ac
Bacteroidetes	Flavobacteriia	Flavobacteriaceae	Chryseobacterium	2
	Sphingobacteriia	Chitinophagaceae	Chitinophaga								T ac

aIdentification based on NCBI (95% limit) and RDP Classifier (80% confidence threshold) results is given, when possible, at genus level.
^bIndividuals positive for the presence of the specific band in the DGGE analysis.
^cThe host tree from which insects were sampled are indicated in brackets: ac: Acer; al: Alnus; li: Liquidambar; sa: Salix.
^dT: testicles; E: immature eggs.
*1 out of 3 ac, and 3 out of 6 al were recovered on LGI medium.