Acid Lipase from Candida viswanathii: Production, Biochemical Properties, and Potential Application

Table 6

Effect of substances on Candida viswanathii lipase stability.

	Relative activity (%)
Substances	10 mM	20 mM
Control (no substance added)	100±4.8	100±4.8
ZnSO4	84.4±3.6	60.0±10.0
MgSO4	99.9±3.9	96.5±4.0
BaCl2	106.7±3.6	98.6±3.7
CoCl2	60.8±7.4	42.7±1.0
NaCl	110.4±5.9	117.4±6.8
NH4Cl	106.8±4.2	104.4±3.74
Pb(CH3COO)2	95.1±5.0	94.2±6.3
CaCl2	103.2±4.4	96.6±4.9
EDTA	103.7±4.2	98.6±6.1
Emulsifiers	1%	5%
SDS	ND	ND
Tween 20	88.2±9.5	52.5±4.9
Tween 80	93.8±8.6	60.5±5.8
Triton X-100	30.3±6.7	26.9±8.7
Gum Arabic	107.3±4.8	102.0±5.1

Assay conditions: lipase activities were assayed on pNPP hydrolysis after 1 h of incubation using McIlvaine buffer pH 3.5, at 40°C, without substrate.