Research Article

CX3CR1 Receptor Polymorphisms, Th1 Cell Recruitment, and Acute Myocardial Infarction Outcome: Looking for a Link

Figure 3

Confocal microscopy examination of triple stain in a culprit lesion (coronary artery from AMI group). (a) (magnification 2000x) shows a 2D reconstruction triple stain for T cells, CD3 (blue), fractalkine receptor CX3CR1 (green), and IFN-gamma (red); yellow, light blue, and white areas were due to multiple positivity. (b) CD3 immunostaining revealed by coumarin conjugated streptavidin; (c) CX3CR1 immunostaining revealed by FITC conjugated streptavidin; (d) IFN-gamma immunostaining revealed by Texas Red conjugated streptavidin.
451349.fig.003