BioMed Research International / 2013 / Article / Fig 5

Research Article

Immunobiologic and Antiinflammatory Properties of a Bark Extract from Ampelozizyphus amazonicus Ducke

Figure 5

Levels of antibody-producing (CD138+) cells in the spleen of P. chabaudi-infected animals treated with SART. Four different groups: CTR, SART, Pc, and Pc + SART were established as detailed in Figure 4. Splenic cells were obtained from individual animals and 106 cells were labeled with anti-B220-FITC and anti-CD138-PE antibodies. Samples were run in a FACScalibur Flow Cytometer. Data shown in (a) indicate a typical profile of CD138+ cells in the B220+ lymphoid population in each experimental group. The percentage of CD138+ cells is included in each graphic. Data in (b) indicate the mean value ± SD of the percentage of CD138+ cells from each group. Six animals were used per group and data are representative of two independent experiments. when compared to the Pc group.

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