Research Article
Structure-Based Mechanism for Early PLP-Mediated Steps of Rabbit Cytosolic Serine Hydroxymethyltransferase Reaction
Table 4
Rabbit cytosolic SHMT structures compared in this study. The table shows the cofactor form and the ligand present in each subunit of the structures.
| Enzyme | Chain | Form | Ligand |
| Unliganded wild type | A | i.a. | | B | i.a. | | C | i.a. | MES | D | i.a. | MES |
| Unliganded T254A | A | i.a. | | B | i.a. | | C | i.a. | | D | i.a. | |
| T254A + glycine | A | g.d. | Gly. | B | g.d. | Gly. | C | g.d. | Gly. | D | g.d. | Gly. |
| T254A + L-serine | A | g.d. | L-Ser. | B | g.d. | L-Ser. | C | g.d. | L-Ser. | D | g.d. | L-Ser. |
| Unliganded T254C | A | i.a. | H2O | B | i.a. | H2O | C | i.a. | H2O | D | i.a. | PO4 |
| T254C + glycine | A | g.d. | Gly. | B | g.d. | Gly. |
| T254C + L-serine | A | g.d. | L-Ser. | B | g.d. | L-Ser. | C | g.d. | L-Ser. | D | g.d. | L-Ser. |
| Wild type + glycine + 5-CHO-H4PteGlu3 | A | g.d. | Gly. | B | i.a. | ā | C | g.d. | Gly. | D | i.a. | ā |
|
|
i.a.: internal aldimine. g.d.: gem-diamine. MES: 2-(N-morpholino)ethanesulfonate. 5-CHO-H4PteGlu3: triglutamic form of 5-formyltetrahydrofolate.
|