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| Primers | Sequence (5′-3′) | Purpose |
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| Hxt7 upstream-HX | CATAGATCTCTCACAAATTAGAGCTTCAATTTAAT | Cloning the fragment of HXT7p-araA-PGKt1 |
| Pgk6 downstream-S | CATGTCGACAGCAATTTAACTGTGATAAACTACCG | Cloning the fragment of HXT7p-araA-PGKt1 |
| Hxt7 upstream-EEB | CATCGGCCGAGATCTCCTAGGCTCACAAATTAGAGCTTCAATTTAAT | Cloning the fragment of HXT7p-araB-PGKt1 |
| Pgk6 downstream-S | CATGTCGACAGCAATTTAACTGTGATAAACTACCG | Cloning the fragment of HXT7p-araB-PGKt1 |
| Hxt7 upstream | CATCCTAGGCTCACAAATTAGAGCTTCAATTTAAT | Cloning the fragment of HXT7p-araD-PGKt1 |
| Pgk6 downstream | CATCCTAGGAGCAATTTAACTGTGATAAACTACCG | Cloning the fragment of HXT7p-araD-PGKt1 |
| HXT7p-F | CCCAAGCTTCTCACAAATTAGAGCTTCAATT | Cloning HXT7p |
| HXT7p-R | ACGCGTCGACATTGGATCTAGATGCATTCGCG | Cloning HXT7p |
| TEF1 W up | CCCAAGCTTCACAATGCATACTTTGTACGTT | Cloning TEF1p |
| TEF1 W down | GCGCGTCGACTTGTAATTAAAACTTAGATTAG | Cloning TEF1p |
| AraA W up | ACGCGTCGACATGTTATCTGTTCCTGATTATG | Cloning araA |
| AraA W down-His | TACGAGTCTTTAGTGGTGGTGGTGGTGGTGTTTTAAAAATGCTTTTGTCA | Cloning araA |
| AraA-F | CAAGCAGGTGGTGGTCATCATAC | For quantitative real-time PCR of araA |
| AraA-R | TACCAACCATTGTAGCGTAATCTTCC | For quantitative real-time PCR of araA |
| AraB-1F | ATGCAGCATTCGCACCTTTG | For quantitative real-time PCR of araB |
| AraB-1R | CCTTCACCTGCTGTGGACAT | For quantitative real-time PCR of araB |
| AraD-1F | CCAGCTGCAGATGCATTAACT | For quantitative real-time PCR of araD |
| AraD-1R | ACAGCCTTAGCTGGTGTTGG | For quantitative real-time PCR of araD |
| Gal2 up | GCTCTAGAATGGCAGTTGAGGAGAACAATATGC | Cloning GAL2 |
| Gal2 down | ACGCGTCGACTTATTCTAGCATGGCCTTGTAC | Cloning GAL2 |
| pG418-Apa I up | AGTGGGCCCTAGGTCTAGAGATCTGTTTAGC | Cloning KanMX4 |
| pG418-Nde I down | GGAATTCCATATGATTAAGGGTTCTCGAGAGCTCG | Cloning KanMX4 |
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