Figure 2: Adherence of Acanthamoeba spp. to MDCK cells. Trophozoites (7.5 × 104) of A. castellanii (grey circle) and A. polyphaga (black diamond) were incubated for different times with confluent MDCK epithelial cell monolayers. Adhesion was evaluated by an ELISA-based assay (absorbance of 7.5 × 104 trophozoites corresponded to 100%). Assays were performed in triplicate.