Figure 6: LCA induced mitochondrial ROS generation in T24 cells. (a) The cells were treated with LCA ( , or 100 μM), and then mitochondrial ROS level was measured using DHR. (b) The cells were treated with LCA (0 or 50 μM) with or without mitochondrial complex I inhibitor rotenone (100 μM) or complex III inhibitor antimycin A (20 μM), and then ROS levels were measured using H2DCFDA. Data are presented as the means   SD of the three independent experiments. , compared with the control group; compared with the LCA group alone (50 μM).