Functional and Molecular Characterization of Ex Vivo Cultured Epiretinal Membrane Cells from Human Proliferative Diabetic Retinopathy

Table 2

Immunophenotyping of the fvERM outgrowing cells and primary hRPE cells. The expression of different groups of surface markers was compared between fvERM and primary hRPE cells. The two cell types showed differential expression of CD14, CD18/integrin β2, CD51/Integrin αV, and CD90/Thy-1. (Data shown represent percentage of positive cells within the total cell culture and are representative of 3 independent experiments on 3 different donor fvERM samples, mean ± SD; 𝑃<0.05^∗, 𝑃<0.01^∗∗).

		fvERM cells	Primary hRPE cells
Hematopoietic	CD11a (LFA-1)	0.00±0.00	0.00±0.00
	CD14	1.81±1.06	66.60±11.26^∗∗
	CD34	21.81±15.78	2.34±1.17
	CD45	0.00±0.00	0.00±0.00
	CD47	97.95±0.44	88.04±5.48
Monocyte markers	CD117/c-kit	0.94±0.76	19.80±16.53
	CXCR4	0.41±0.25	7.28±5.22
	HLA-DR	0.08±0.08	1.00±1.00
	HLA-G	0.00±0.00	0.00±0.00
	CD338 (ABCG2)	0.80±0.08	17.63±15.09
MSC; Fibroblast markers	CD73	98.37±0.32	76.55±22.76
	CD90/Thy-1	68.19±0.46	91.16±6.66^∗
	CD105/Endoglin	0.29±0.29	23.10±11.60
	PDGF Rβ	36.46±14.11	56.59±7.66
CAMs; Integrins	CD18 (Integrin β2)	0.25±0.17	69.86±16.38^∗
	CD29/Integrin β1	98.34±0.48	98.38±1.40
	CD31/PECAM	0.00±0.00	7.60±6.52
	CD44/H-CAM	96.78±1.06	89.03±6.71
	CD49a/Integrin 𝛼1	6.06±3.53	50.36±25.67
	CD49b/Integrin α2	0.11±0.07	49.30±25.56
	CD51 Integrin αV	21.07±9.14	85.98±5.54^∗∗
	CD54/ICAM-1	32.65±5.45	52.47±10.83
	CD106/V-CAM 1	4.17±2.60	6.43±3.85
	CD146/MCAM	5.91±5.69	24.58±23.52
	CD166/ALCAM	95.70±1.64	95.28±4.47