Characterization of Cardiac-Resident Progenitor Cells Expressing High Aldehyde Dehydrogenase Activity
Figure 1
Identification of cells in populations isolated from whole hearts from neonatal and young adult mice. Flow cytometric analysis of ALDH activity (a). Neonatal mice. Left panel: cells were selected according to forward scatter (FSC) and side scatter (SSC) properties using the gated region. Middle panel: cells incubated with Aldefluor substrate and the specific inhibitor of ALDH, DEAB were used to establish baseline fluorescence of these cells and to define the (R2) and the (R3) region as less than 0.1% and 0% of total events, respectively. Right panel: cell incubation with Aldefluor substrate in the absence of inhibitor induced a shift in FL1 fluorescence defining the (R1), the (R2), and the (R3) populations (b). Eight-week-old mice (c). Bar histogram showing and cell percentages (mean, SD) in neonatal and young adult mice. value refers to the difference between neonatal and 8 week-old mice.
