Characterization of Cardiac-Resident Progenitor Cells Expressing High Aldehyde Dehydrogenase Activity
Differentiation potential of expanded cells. (a) Photomicrograph showing intracellular fat droplets in cells cultured in adipogenic medium. (b) Positive Oil Red-O staining of intracellular droplets. (c) Positive type-II collagen immunostaining (green) in cells cultured in chondrogenic medium. (d) Type-II collagen immunostaining of leg cartilage, as a positive control. (e) Positive sarcomeric α-actinin immunostaining (green) of an cell (the protein does not show clear sarcomeric distribution). (f) Corresponding negative control (secondary Ab only). (g) Positive Alizarin Red staining of expanded cells cultured in osteogenic medium.