Brazilian Cerrado Soil Actinobacteria Ecology

Table 2

Actinobacterial-specific primer used in bacterial communities in the Brazilian Cerradosoils, according to Schäfer et al. [12].

Primer	Sequence (5′–3′)	Primers	Amplified fragment (bp)	PCR conditions
27f	GAG TTT GAT CMT GGC TCA G	Bacterial universal primer	~1500	Denatured for 5 min at 95°C. 30 cycles: denaturing at 92°C for 60 s, annealing at 55°C for 60 s, and extension at 72°C for 60 s; final extension for 10 min at 72°C
1492r	ACG GYT ACC TTG TTA CGA CTT
Com2xf	AAA CTC AAA GGA ATT GAC GG	Actinobacterial-specific primer	~270
Ac1186r	CTT CCT CCG AGT TGA CCC
SC-Act-235aS20	CGC GGC CTA TCA GCT TGT TG	Actinobacterial-specific primer	~640
SC-Act-878aA19	CCG TAC TCC CCA GGC GGG G