Systems Approaches to Modeling Chronic Mucosal Inflammation
Figure 1
TGFβ induces EMT transition in HSAECs. (a) F-actin staining. Shown in green is a confocal microscopic image of FITC-phalloidin staining merged with DAPI staining (blue) of HSAECs, A549 cells, and HBECs in the absence or presence of TGFβ stimulation as indicated. (b) Expression of EMT program. Shown is normalized mRNA expression in HSAECs in the presence or absence of EMT after 10 d of TGFβ treatment. For each gene, mRNA expression was normalized to β-pol as a housekeeping gene and expressed as the fold change relative to its expression in the absence of TGFβ stimulation. Shown is mean and SE of replicate measurements. Each point is the mean of a duplicate biological experiment, measured with three technical replicates. Abbreviations: SLUG, Snail 2; SNAL1, Snail 1; col1A, collagen type 1a.
