Table 1: Dilution scheme used for the assay of conjugative action of SL and tetracycline against S. aureus.

Sr. no.Test reaction descriptionVolume of SL stock ( L)Volume of antibiotic stock ( L)Volume of sterile distilled water ( L)Volume of bacterial suspension ( L)Total volume
( L)

1Control8002001000
2SL alone307702001000
3SL + tetracycline30157552001000
4Tetracycline157852001000