The Effect of Conditioned Media of Adipose-Derived Stem Cells on Wound Healing after Ablative Fractional Carbon Dioxide Laser Resurfacing
(a) Overall elastin (R2) after fractional carbon dioxide (CO2) laser resurfacing. T1 and T2 are ADSC-CM-treated sites after a single pass of fractionated CO2 laser energy of 8 and 16 mJ, respectively. C1 and C2 are the DMEM-applied control sites after single passes of 8 and 16 mJ, respectively. D0: before treatment; D1: 1 day after treatment; D3: 3 days after treatment; D7: 7 days after treatment; D14: 14 days after treatment; and D21: 21 days after treatment. (b) HE (1 and 2), Masson-Trichrome (3 and 4), and Gomori’s aldehyde fuchsin (5 and 6) staining of biopsy specimen from fractional carbon dioxide laser-treated sites on day 21. Scale bar 50 μm. (c) Total RNA was extracted from biopsied skin samples of different groups. Procollagen types I and III and elastin mRNA were determined by real-time RT-PCR analysis. Results are shown as means ± SD (). The asterisk (*) indicates a significant difference () between the control group and the ADSC-CM-treated group.