Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of Phlebotomus perniciosus from Madrid, Spain

Figure 2

Multiple sequence alignment of D7-related proteins from sand flies and other related sequences: L. longipalpis (Lon), L. intermedia (Int), L. ayacuchensis (Aya), P. arabicus (Ara), P. ariasi (Ari), P. perniciosus Italian strain (PerIta), P. perniciosus Spanish strain (PerSpa), P. tobbi (Tob), P. argentipes (Arg), P. duboscqi (Dub), P. papatasi (Pap), P. sergenti (Ser), A. stephensi (Ans), and A. aegypti (Aed). Accession numbers are indicated in the sequence name. Sequences without a signal peptide were aligned with ClustalW and refined using Boxshade server, and the percentage of the identities or similarities that must agree for shading was set at 80%. Black background shading represents identical amino acids, and grey shading designates similar amino acids, while white shading indicates no similarity. (*) indicates changes in the prediction of the antigenic index between P. perniciosus Spanish and Italian strains as performed by Protean (DNASTAR, Lasergene). (♠) denotes conserved cysteine residues. The cysteinyl leukotriene-binding motif is indicated by (□) [42], and the mutation of one of the amino acids that integrate this motif is designated by (▼). Tyrosine in position 52 in A. stephensi stabilizes TXA2 analogs and is highlighted with (○).